Decreased Efficiency of Free Naked DNA Transformation by Chlorine and UV Disinfection and Its Detection Limitations

Antibiotic resistance genes can be spread via gene horizontal transfer (GHT). Chlorination and UV irradiation are common disinfection methods used in wastewater treatment plants before the discharge of treated wastewater. This study aimed to elucidate effects on transformation naked DNA aquatic environment. The pUC19 plasmid possessing ampicillin-resistant beta-lactamase subjected different dosages chlorine or was for Escherichia coli estimate efficiency GHT environment after disinfection. results showed that doses > 0.5 mg-Cl2/L effectively decrease (1.21 8.83-log10) based as positive control. (2.37 3.39-log10) following 10–60 min treatment. PCR qPCR detection have limitations determining because they provide approximate estimates damaged DNAs. Overall, these indicate proper management using and/or from prevent antibiotic resistant bacteria genes, by decreasing naturally occurring bacterial transformations plants.